Ba-Hoanh Nguyen, Duc-Dien Nguyen and Le-Ai-Vinh Nguyen.
Soybean (Glycine max
(L.) Merr. cv. “Nam Dan”) is one of the most valuable crops in
agricultural production in Nghe An province (Vietnam). Our previous
study revealed that extract of the cyanobacterium strain Nostoc calcicola
HN9 expressed positive effect on growth and development, and raised
soybean productivity (Tran et al. in Proceeding of Vietnam national
conference of research on biology, Da Nang, 2016). We hypothesized that N. calcicola HN9 would improve the defense responses of G. max cv. “Nam Dan” to cowpea aphid (Aphis craccivora Koch)-a serious pest of leguminous crops.
Infestation of A. craccivora caused oxidative stress in leaves of G. max cv. “Nam Dan”. A strong generation of endogenous reactive oxygen species (ROS) such as superoxide anion radical (O2·−) and hydrogen peroxide (H2O2)
resulted in the cellular damages in the aphid-infested leaves through
high levels of injury percentage and lipid peroxidation. To protect from
aphid attack themselves, soybean plants triggered the antioxidant
defense systems, in which, enzymatic antioxidants such as superoxide
dismutase (SOD, 18.104.22.168), catalase (CAT, 22.214.171.124) and GPx (EC
126.96.36.199) were strongly accumulated to reduce the toxic effects of ROS.
Components of N. calcicola HN9 extract might strengthen the defensive capability of G. max
cv. “Nam Dan” to cowpea aphid infestation via establishing the chemical
constraints on oxidative stress. Under effect of cyanobacteria extract,
generation of O2·− and H2O2
was strictly limited, activities of SOD, CAT and GPx were remarkably
accumulated in the aphid-infested leaves leading to a significant
reduction of oxidative damages.
Nostoc calcicola HN9 extract probably not only controlled the generation and effects of O2·− and H2O2
but also augmented the accumulated activity of SOD, CAT and GPx in
soybean leaves that allowed them to control oxidative stress,
contributed to increase the resistance of G. max cv. “Nam Dan” to A. craccivora.
The improvement of cyanobacteria extract on the antioxidative response
of soybean “Nam Dan” to cowpea aphid can be a novel aspect to contribute
to current knowledge regarding the soybean-aphid interaction.
- Keywords: Aphis craccivora, Glycine max, Nostoc calcicola HN9, Hydrogen peroxide, Superoxide anion radical, Catalase, Glutathione peroxidase, Superoxide dismutase.
The article was published on: https://doi.org/10.1186/s40529-017-0211-9